Determination of ploidy inCryptococcus neoformansby flow cytometry
نویسندگان
چکیده
منابع مشابه
determination of ploidy levels of some populations of agropyron cristatum (poaceae) in iran by flow cytometry
flow cytometry (fcm) was used to determine the ploidy levels of six different populationsof agropyron cristatum (l.) gaertn. (poaceae) in iran, using a diploid cultivated barley (hordeumvulgare l.) as an internal reference. according to flow cytometric data, tetraploid (2n = ± 28) andhexaploid (2n = ± 42) levels were detected among the examined populations and these results weresupported by chr...
متن کاملComparison of flow and static image cytometry in the determination of ploidy.
AIMS To compare the efficiency of flow cytometry and computed image cytometry; and to see if a reliable set of guidelines regarding interpretation of histograms could be drawn up. METHODS The two methods were applied to a series of 111 formalin fixed renal cell carcinomas. Data generated by both methods were compared. RESULTS The methods agreed in 85 cases. Hypodiploidy was detected by comp...
متن کاملFlow cytometry (ploidy determination, cell cycle analysis, DNA content per nucleus)
During recent years, flow cytometry has established itself as a useful, quick novel method to determine efficiently, reproducibly and at a reduced cost per sample the relative nuclear DNA content and ploidy level of a large number of species (plants and animals), that has also been used to sort cells with different traits from a mixed cell population (e.g. as when separating heterokaryons from ...
متن کاملEstimation of Nuclear DNA Content and Determination of Ploidy Level in Tunisian Populations of Atriplex halimus L. by Flow Cytometry
Kheiria Hcini1, David J. Walker2, Elena González3, Nora Frayssinet4, Enrique Correal2 and Sadok Bouzid1 1Faculté des Sciences de Tunis, Département des Sciences Biologiques, Tunis, 2Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor, s/n 30150 La Alberca, Murcia, 3Facultad de Ciencias Exactas y Naturales, UNPSJB – Comodoro Rivadavia, 4Facultad de Agronomía, ...
متن کاملEfficient Doubled Haploid Production in Brassica napus via Microspore Colchicine Treatment in vitro and Ploidy Determination by Flow Cytometry
An efficient microspore culture protocol was used to rapidly produce homozygous doubled haploid (DH) progeny of crosses between low erucic canola and high erucic resynthesized rapeseed (Brassica napus L.). Microspores of Canadian cultivars ‘Excel’ and ‘Profit’ as well as three F1 hybrids with the resynthetic line RS239 were treated with colchicine immediately after isolation. Flow cytometry was...
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ژورنال
عنوان ژورنال: Medical Mycology
سال: 1996
ISSN: 1369-3786,1460-2709
DOI: 10.1080/02681219680000521